Thedevelopmentofinnovativeskincareproductsrequiresadvanced,human-relevantinvitrotestingtechnologies.WhenworldleADIngpersonalcareandcosmeticscientistsevaluatenovelingredientsandformulations,theychoosetheEpiDermFT3Dhumanskinmodel.
Engineeredtoenableinvitroskinresearchinwhichfibroblast-keratinocytecellinteractionsareimportant,MatTek’sEpiDermFTsystemconsistsofnormal,humanepidermalkeratinocytes(NHEK)andnormal,humandermalfibroblasts(NHFB)culturedtoformamultilayeredmodelofthehumandermisandepidermis.Culturedattheair-liquidinterfaceineasy-to-handletissuecultureinserts,EpiDermFTattainslevelsofdifferentiationatthecuttingedgeoftissueengineeringtechnology.
EpiDermFTconsistsoforganizedkeratin5expressingbasalcells,involucrinandkeratin10expressingspinousandgranularlayers,andcornifiedepidermallayersanalogoustothosefoundinvivo.Thedermalcompartmentiscomposedofacollagenmatrixcontainingviablenormalhumandermalfibroblasts(NHDF).Theepidermalanddermallayersaremitoticallyandmetabolicallyactiveandexhibitinvivo-likemorphologicalandgrowthcharacteristicswhichareuniformandhighlyreproducIBLe.
Awell-developedbasementmembraneispresentatthedermal/epidermaljunction.Hemidesomosomes(›),laminalucida,laminadensa(→)andanchoringfibrilstructures(»)areevidentbytransmissionelectronmicroscopy.
EpiDermFTtissuesareusedacrossawiderangeofapplicationsincludingscientificefficacyandclaimssubstantiation.Duetoadryskinsurface,EpiDermFTcanbeusedtoevaluateBIOLOGicalresponsestotopicalapplicationsofformulationsoringredients.Simpleprotocolsandtheevaluationofearlymolecularendpointsallowresearchtoacquiredataindays,notweeksormonths.
Examinechangesinextracellularmatrixgeneandproteinexpressionandhistologicalstructuretodeterminetheanti-agingefficacyofcosmeticingredientsorfinalformulation.EpiDermFTisavailablefromarangeofadultdonors.
Anti-AgingApplicationNote
UseEpiDermFTtoevaluatetherapeuticswiththewoundhealingassaykit.Woundhealingassaykitsinclude24pre-woundedtissuesandareavailablewithepidermalonlywoundsorfullthicknesswoundsoftheepidermisanddermis.
RecentWoundHealingPoster
UseEpiDermFTtoassesstheefficacyoftopicallyappliedmoisturizersbymeasuringtheelectricalimpedanceoftheepidermis.Amenabletocreams,gel,lotionsorotherformulations,theSkinHydrationassayallowsfordataanalysiswithin24hours.
SkinHydrationApplicationNote
Analyzecyclopyrimidinedimerformation,inflammatorymediatorreleaseandoxidativeendpointstoevaluatetheUVprotectiveefficacyofcompoundsorformulations.
UVProtection ApplicationNote
BrowseourreferencelibrarytoseehowourEpiDermFT tissuehasbeenusedintheseareasofstudy.
Kit: AstandardEpiDermFTkit(EFT-400)consistsof24tissues.(Tissue“kits”containtissues,asmallamountofculturemedium,andplasticware;contactMatTekforspecifickitcontents)
Substrate: CostarSnapwell™singlewelltissuecultureplateinserts areused.Poresize=0.4μm,Diameter=1.2cm.Surfacearea=1.0cm2
Culture: Air-liquidinterface
Histology: 8-12celllayersplusstratumcorneum(basal,spinous,andgranularlayers)
Lotnumbers: Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualkitswithinagivenlotoftissues.Alltissuekitswithinalotareidenticalinregardstocells,medium,handling,cultureconditions,etc.
Shipment: At4°Conmedium-supplemented,agarosegelsin6-wellplates
Shipmentday: EveryMonday.ShipmentonThursdayalsopossibleuponspecialrequest
Delivery: TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-Thursdaydependingonlocation
Shelflife: Includingtimeintransit,tissuesmaybestoredat4°Cforupto6dayspriortouse.However,extendedstorageperiodsarenotrecommendedunlessnecessary.Inaddition,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning)
Lengthofexperiments: Culturescanbecontinuedforupto2weekswithgoodretentionofnormalepidermalmorphology.Culturesmustbefedeveryotherdaywith5.0mlofEFT-400medium
Type: Normalhumanepidermalkeratinocytes(NHEK);Normalhumandermalfibroblasts(NHDF)
Geneticmake-up: Singledonor
Derivedfrom: Neonatal-foreskintissue(NHEK);Neonatalskin(NHDF)
Alternatives: NHEKfromadultbreastskin;NHDFfromadultskin
Screenedfor: HIV,Hepatitis-B,Hepatitis-C,mycoplasma
Basemedium: Dulbecco’sModifiedEagle’sMedium(DMEM)
Growthfactors/hormones: Epidermalgrowthfactor,insulin,hydrocortisoneandotherproprietarystimulatorsofepidermaldifferentiation
Serum: None
Antibiotics: Gentamicin5µg/ml(10%ofnormalgentamicinlevel)
Anti-fungalagent: AmphotericinB0.25µg/ml
pHIndicator: Phenolred
Otheradditives: Lipidprecursorsusedtoenhanceepidermalbarrierformation(proprietary)
Alternatives: Phenolred-free(EFT-400-PRF),antibiotic-free(EFT-400-ABF),anti-fungal-free(EFT-400-AFF),orhydrocortisone-freemediumandtissue(EFT-400-HCF)areavailable.Agentsareremovedatleast3dayspriortoshipment
Assay/Maintenancemedium: EFT-400-ASYisutilizedforassays;EFT-400-MMisusedfortermmaintenanceoftheEFT-400tissues
Visualinspection: Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipment
End-usetesting: Tissuesareexposedto1%TritonX-100for8,12,16and24hours.ThetimeofexposurerequiredtoreducethetissueviABIlity(ET-50)usingtheMTTviabilityassayisdetermined(SeeMatTekEpiDermFTuseprotocol)foreachlotoftissue.ET-50’sgenerallyfallwithintherangeof6.5-9.5hours.ET-50’sincustomers’labmaydifferslightlyfromtheMatTekresults
Sterility: Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontamination
Screeningforpathogens: AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“Biosafetyinmicrobiologicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalservice
Notificationoflotfailure: IfatissuelotfailsourQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutchargewhenappropriate.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossible(Undernormalcircumstances,ET-50failureswillbenotifiedbyWednesday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)
25 年来,MatTek 一直是可靠的体外人体组织模型创新的全球领导者。我们的皮肤、眼部、口腔、呼吸道和肠道组织模型用于评估化妆品、化工、制药和家居用品行业的安全性和有效性。这些先进的组织模型使公司能够实现非动物测试的目标,同时降低测试成本并提供与人类相关的结果。
随着 MatTek 培养皿、原代人类细胞和培养基、3Diy 试剂盒以及为寻求体外解决方案的客户提供测试服务,MatTek 从一家组织工程公司扩展到一家完整的生命科学公司。行业领先的 MatTek 培养皿可在标准尺寸的细胞培养皿和培养板中提供出色的成像。我们在细胞分离和扩增方面的专业知识使我们能够为二维研究开发最高质量的原代人类细胞和培养基。我们的 3Diy 试剂盒通过为研究人员提供以较低成本在 3D 组织模型中使用特化细胞的机会,进一步弥合了 2D 细胞培养和即用型 3D 组织之间的差距。我们在体外科学领域拥有无与伦比的专业知识,并拥有通过我们的测试服务指导客户获得可靠结果的知识。